Exosome Cargo From Preeclampsia Patients

Inclusion 1. Preeclampsia patients: Age ≥ 18 years; Diagnosis of preeclampsia with severe features: 1. BP ≥160/110 after 20 weeks gestation AND ≥300 mg/day proteinuria or protein/creatinine ratio of 0.3 mg/dL; OR 2. BP ≥160/110 after 20 weeks gestation with any of the following co-conditions: platelet count less than 100,000 X 109/L, AST/ALT enzymes elevated to twice the upper limit of normal, serum creatinine ≥1.1 mg/dL or a doubling of the creatinine from baseline, pulmonary edema, new-onset headache, and/or visual disturbances. 2. Control patients: Age ≥ 18 years; no diagnosis of preeclampsia or any pregnancy-induced hypertension disorder. 3. Pre-clampsia patients will be defined as =140 mmHg systolic or =90mmHg diastolic BP with at least at least 2 occasions 4 hours apart after 20 weeks of gestation in previously normotensive women with at least one of the following symptoms: =300 mg/day proteinuria, protein/creatinine ratio of 0.3 mg/dL, platelet count \< 100,000 X 109/L, elevated liver enzymes, serum creatinine =1.1 mg/dL, pulmonary edema, or new-onset headache or visual disturbances. 1. \>22w 0d gestational age -33w 6d GA (n=16) 2. \>= 34 wGA (n=16) 4. Pre-eclampsia with severe features will be defined as blood pressure =160 mmHg systolic or =110 mmHg diastolic BP at least 2 occasions 4 hours apart after 20 weeks of gestation with any of the additional diagnostic criteria listed above. 1. \>22w 0d gestational age -33w 6d GA (n=16) 2. \>= 34 wGA (n=16) 5. Healthy gestational age matched controls: 1. \>22w 0d gestational age -33w 6d GA (n=32) 2. \>= 34 wGA (n=32) Exclusion criteria: 1. Preeclampsia patients: Age \< 18 years; Any other diagnosis of pregnancy-induced hypertension that isn't preeclampsia with severe features (i.e. gestational hypertension or preeclampsia without severe features). 2. Control Patients: Age \< 18 years; Any diagnosis of pregnancy-induced hypertension.