Short-term Fat Overfeeding on the Effects of Liver Metabolism

Inclusion Criteria: * The participant is willing and able to give informed consent for participation in the study. * Male or Female, aged ≥18 or ≤65 years. * Body Mass Index ≥19 ≤35 kg/m2 * No medical condition or relevant drug therapy that is known to affect liver or adipose tissue metabolism. * Weight stable for the previous 3 months Exclusion Criteria: * The participant is unwilling or unable to give informed consent for participation in the study. - Aged ≤18 or ≥65 years * Body Mass Index ≤19 or ≥35kg/m2 * Blood haemoglobin \<135mg/dL for men and \<120mg/dL for women * Donated (or lost) ≥250 ml of blood in the previous two months. * On a weight loss diet or have decreased their body weight by \>5% in the previous 3 months. * Have increased their body weight by \>5% in the previous 3 months. * Any metabolic condition or relevant drug therapy * Current smoker * History of alcoholism or a greater than recommended alcohol intake (\>30 g of alcohol daily for men and \>20 g of alcohol daily for women) * Haemorrhagic disorders * Anticoagulant treatment * History of albumin allergy * Pregnant or nursing mothers * Women prescribed any contraceptive agent or device including oral contraceptives, hormone replacement therapy (HRT) or who have used these within the last 12 months History of severe claustrophobia * Presence of metallic implants, pacemakers, or are unwilling to remove any piercings * History of an eating disorder or any other psychological condition that may affect the participant's ability to adhere to study intervention/experimental diets.