Disentangling the Effects of Daily Stress, Sleep, and Sex Hormones on Accelerated Vascular Aging in Midlife Women

Inclusion Criteria: * Female * Age 40-55 * Premenopausal Exclusion Criteria: * Pregnancy (including a positive urine pregnancy test), planning to become pregnant, or breast-feeding * Peri- or post-menopausal * Unstable or diagnosed chronic clinical disease, including cardiovascular, metabolic, renal, hepatic, autonomic, autoimmune, or dermatological disease * Current or lifetime major psychiatric illness (e.g., major depressive disorder, bipolar disorder, schizophrenia, eating disorders) * History of hysterectomy (complete or partial), polycystic ovary syndrome, endometrial hyperplasia, irregular menstrual cycles, or endometrial ablation * Suspected potential presence of obstructive sleep apnea (STOP-Bang score \>3) or insomnia (Insomnia Severity Index score \>15) * Current or recent (within 8 wks) use of medications that could conceivably alter cardiovascular function or sleep health * Changes or alterations in medication status (starting a new, additional, or different medication or changing the dose of a current medication) * Body mass index \>35 kg/m2 * Tobacco or nicotine use, including vaping and electronic cigarettes * Working night-time or rotating shift work within the previous 3 months * Trans-meridian travel during study enrollment (inclusive of the entire duration of enrollment and the period between testing cycles) * Known allergies to pharmacological agents/drugs * Current use of long-acting reversible contraception (LARC; e.g., IUDs, birth control implants) * Known latex allergy