Insomnia´s Impact on DNA Stability, Cardio-metabolic Health and Lifestyle Behaviors

Inclusion Criteria: Each population group will meet the following inclusion criteria: * Healthy Individuals (18-35 years old, men and women) * Waist circumference: \<90 cm (men), \<80 cm (women) * Fasting glucose: 70-110 mg/dL * Blood pressure: \<120/80 mmHg * Triglycerides: \<150 mg/dL * HDL-C: \>40 mg/dL (men), \>50 mg/dL (women) * Physical activity level: 600-1500 MET-min/week * Athletes (18-35 years old, men and women) \*Engaged in structured sports training with a physical activity level \>1500 MET-min/week * Older Adults (65-75 years old, men and women) \*Free of cognitive or mental health disorders. No specific cardiometabolic or body composition criteria due to the study's exploratory nature. * University Students (18-35 years old, men and women) \*No specific cardiometabolic or body composition criteria due to the study's exploratory nature. * Individuals with Metabolic Syndrome (18-35 years old, men and women) * Waist circumference: \>90 cm (men), \>80 cm (women) * Fasting glucose: \>110 mg/dL * Blood pressure: \>130/80 mmHg * Triglycerides: \>150 mg/dL * HDL-C: \<40 mg/dL (men), \<50 mg/dL (women) * Physical activity level: \<600 MET-min/week Exclusion Criteria: * Individuals with respiratory diseases or musculoskeletal injuries that prevent exercise, as determined by a clinical history review, a physical activity readiness questionnaire (PART-Q+), and pulmonary function tests (spirometry). Individuals with rotating or night-shift work schedules (e.g., emergency personnel, security guards), as circadian rhythm disruptions significantly impact sleep quality * Women diagnosed with polycystic ovary syndrome (PCOS) or showing high testosterone levels with a history of oligomenorrhea, as these conditions may influence metabolic and hormonal markers. However, women with menstrual irregularities related to premenstrual syndrome (PMS) or dysmenorrhea will not be excluded, given the established link between sleep disorders and menstrual cycle variations * This study does not involve chronic dietary or exercise interventions, nor does it require long-term participant follow-up, so no additional exclusion criteria apply