Effects of Acute Sleep Deprivation on Individuals With Different APOE Genotypes

Inclusion Criteria: * Age 18-40 years, gender not limited * Healthy (with no clinically significant abnormal findings in the physical examination report or self-reporting as healthy) and not on medications * Cognitively normal (Mini-Mental State Examination (MMSE) score \> 28) * Sleep duration of 7-9 hours per night, good sleep quality (Pittsburgh Sleep Quality Index (PSQI) ≤ 5 points) * Written informed consent, voluntarily participate in this study, and be able to cooperate with the physician to complete the clinical study Exclusion Criteria: * Presence of day-night sleep reversal * Shift work within the past 6 months * Travel across time zones or experience of jet lag within the past three weeks * Current smoking or nicotine use; alcohol consumption exceeding five standard units per week (one standard alcohol unit is defined as 10 mL \[or 8 g\] of pure alcohol) * Consumption of strong tea, coffee, or caffeine-containing foods and beverages within one week before study participation * Family history of early-onset dementia * Self-Rating Depression Scale (SDS) score ≥ 53, Self-Rating Anxiety Scale (SAS) score ≥ 50 * Female participants who are currently pregnant or breastfeeding * Individuals who need to drive or operate vehicles or machinery during the study period