Effect of Dietary Nitrate on Immobilization-induced Changes in Skeletal Muscle in Young Healthy Men

Inclusion Criteria: * Men age 18-44 * Above the minimum threshold of the IPAQ questionnaire * Below the maximum threshold of the IPAQ questionnaire Exclusion Criteria: * Men and women \<18 or \>44 years of age * Unable to provide informed consent * Known clotting disorder * Previous history of deep vein thrombosis * Injury to either leg resulting in reduced mobility in the previous year * Currently dieting or weight instability for the past 3 months * Epileptic * Pacemaker or other implantable heart device * Currently taking antibiotics * Current smoker * Stage II hypertension (resting blood pressure \>140/\>90) * Previously undergone a revascularization procedure involving a vascular graft or stenting of the femoral or popliteal arteries * Those taking phosphodiesterase inhibitors (e.g., Viagra), proton pump inhibitors, antacids, xanthine oxidase inhibitors, hormonal contraceptives or on hormone replacement therapy * An answer of yes to any of the seven questions on the first page of the Physical Activity Readiness Questionnaire (PAR-Q. These exclusions include the following: * If participant's doctor has ever said that he/she has a heart condition and that he/she should only do physical activity recommended by a doctor * Pain in chest when doing physical activity * In past month, chest pain when not doing physical activity * If participant has ever lost balance because of dizziness or has ever lost consciousness * Muscle, bone, or joint problem that could be made worse by physical activity * Currently on prescribed drugs for blood pressure or heart condition. * If the participant knows of any other reason he/she should not do physical activity.