Pharmacokinetics and Pharmacodynamics of Synthetic Nicotine and Nicotine Analogues

Inclusion Criteria: * Men or women, age 18 or older at screening who have used EC with nicotine during at least 10 of the past 30 days * Saliva cotinine concentration of \> 30 ng/mL at screening * Ability to communicate well with the investigator and to understand and comply with the requirements of the study * Women of child-bearing age engaging in sexual activities which can lead to pregnancy: willingness of using a reliable/hormonal contraception method during the study (hormonal, e.g. pill, intrauterine devices, or mechanical method, e.g. condom, diaphragm) * Signed informed consent form Exclusion Criteria: * Known hypersensitivity/allergy to a content of the e-liquids * Smoking of more than 5 cigarettes per day in the past 30 days * Pregnant or lactating women * Intention to become pregnant during the course of the study * BMI \< 18 or \> 30 kg/m2 at screening * History or clinical evidence of alcoholism or drug abuse within the 3-year period prior to screening * Loss of ≥ 250 mL of blood within 3 months prior to screening, including blood donation * Treatment with an investigational drug within 30 days prior to screening * Treatment with prescribed or over-the-counter medications with known influence on CYP2A6 function within 1 week prior to screening (with the exception of contraception) * History or clinical evidence of any disease (e.g. gastrointestinal tract-disease) and/or existence of any surgical or medical condition, which in the opinion of the investigator might interfere with the absorption, distribution, metabolism or excretion of the study drugs, or which might increase the risk of toxicity. * Legal incapacity or limited legal capacity at screening * Any circumstances or conditions, which, in the opinion of the investigator, may affect full participation in the study or compliance with the protocol