Deciphering the Effect of Moderate Wine Consumption on Healthy Aging Through Postprandial Extracellular Vesicles.

Inclusion Criteria * Healthy adult men and women aged 35 to 65 years. * Body Mass Index (BMI) between 18.5 and 29.9 kg/m². * Non-smokers or ex-smokers for at least 12 months. * Moderate alcohol consumers, defined as ≤2 units/day for men and ≤1 unit/day for women. * Normal fasting glucose and lipid profile at screening. * Willing and able to refrain from alcohol, polyphenol-rich foods, and intense exercise for 48 hours before each study visit. * Able to understand the study procedures and provide written informed consent. Exclusion Criteria * History or clinical evidence of cardiovascular, hepatic, renal, thyroid, gastrointestinal, or metabolic diseases (including diabetes, dyslipidemia, or hypertension). * Use of medications or supplements known to affect glucose, lipid, or inflammatory metabolism (e.g., statins, corticosteroids, anti-inflammatory drugs). * Pregnancy or breastfeeding. * Recent blood donation (within the last 3 months) or planned blood donation during the study period. * Major weight change (\>5% of body weight) within the last 3 months. * Participation in another clinical or biomedical study within the previous 3 months. * Known allergy or intolerance to wine, alcohol, or its components (e.g., sulfites). * History of alcohol abuse or inability to abstain from alcohol outside the study context. * Reluctance to receive information about incidental health findings arising from the study. * Any condition judged by the investigators to limit compliance or increase study risk (e.g., psychiatric disorders, inability to adhere to fasting requirements).