Development of Magnetic Resonance Imaging Techniques for Studying Mood and Anxiety Disorders

* INCLUSION CRITERIA: Healthy Controls * Male and female subjects between 18 and 65 years of age * Subjects must be able to give written informed consent prior to participation in this study. * Subjects who do not currently meet and have never met criteria for any major psychiatric disorder, and who have no known first degree relatives with mood disorders. * For cognitive experiments utilizing language stimuli only native English speakers will be enrolled. Major Depressive Disorder * Male and female subjects between 18 and 65 years of age. * Subjects have been found eligible for other ETPB research protocols according to 01-M-0254. * Subjects must fulfill DSM-IV or V criteria for Major Depression based on clinical assessment and confirmed by a structured diagnostic interview (SCID-P). * Subjects must be able to give written informed consent prior to participation in this study. * For cognitive experiments utilizing language stimuli, only native English speakers will be enrolled. EXCLUSION CRITERIA: Healthy Control * Subjects with major medical or neurological disorders expected to influence cerebral blood flow or morphology, or taking any medication that is likely to influence the imaging parameters-of-interest within 3 weeks of scanning. * Women who are pregnant are excluded from the study. Subjects will undergo pregnancy testing no more than 24 hours prior to MRI scanning. * Subjects with contraindication to MRI scanning such as aneurysm clips, implanted neural stimulator, implanted cardiac pacemaker or auto-defibrillator, cochlear implant, or ocular foreign body. * A history of drug or alcohol abuse within 1 year or a lifetime history of drug or alcohol dependence (DSM-IV) or alcohol use disorder (DSM-V equivalent). Major Depressive Disorder * Presence of Axis 1 psychiatric diagnosis other than Major Depression or an Axis 2 disorder * Subjects with major medical or neurological disorders expected to influence cognitive function or are taking any drugs likely to affect mood or cognitive function within 1 week of study participation. Depressed subjects will not be tapered/withdrawn from medications under this study. * A history of drug or alcohol abuse within 1 year or a lifetime history of drug or alcohol dependence (DSM-IV) or alcohol use disorder (DSM-V equivalent). * Subjects with major medical or neurological disorders expected to influence cerebral blood flow or morphology. * Women who are pregnant or breastfeeding will be excluded from MRI portions of the study. Subjects will undergo pregnancy testing no more than 24 hours prior to MRI scanning. * Subjects with contraindication to MRI scanning such as aneurysm clips, implanted neural stimulator, implanted cardiac pacemaker or auto-defibrillator, cochlear implant, or ocular foreign body.