Adverse Adolescent Pathways to Substance Use

Inclusion Criteria: * Male or female * 12-14 years old, * Report symptoms of anxiety * Understand and sign an assent, and parents will sign a permission and a consent document in English * Meets study hearing and vision requirements * Substance use naïve Exclusion Criteria: * Has any foreign metal objects or implants as determined by the safety questionnaires (e.g., bone screws, shunts or body piercing that can't be removed, etc.) * Has been diagnosed with psychosis or a severe emotional disturbance. * Has used alcohol, tobacco, or marijuana more than 3 times. * Is pregnant. Girls who self-report pregnancy may not participate in the stress evaluation or the MRI scan. * Is taking any medications that directly alter cardiovascular function (e.g., propranolol or other beta blockers). * Any current or lifetime treatment with antipsychotic medication. * Has had a head injury that resulted in a loss of consciousness for more than 5 minutes. * Is planning to get non-removable metal braces. * Impaired intellectual functioning (full-scale intelligence quotient \<70). * Past or current history of a clinically significant central nervous system that could confound brain imaging evaluations.